MOC 5005 : Proteins in Industry (3C)

MOC 5005 : Proteins in industry (45 L) - 3 credits

Protein function (14 L)

Active site residues of different enzymes and positioning, Functions of coenzymes, Mechanism of enzyme catalysis, Enzyme catalysis in organic media, Primary and secondary isotope effects on enzyme catalyzed reactions, Enzyme catalysis group transfer reactions: Peptidases, esterases and lipases, Amination,  Phosphorylation reactions catalyzed by enzymes; Phosphatases and phosphodiesterases, kinases, Redox reactions that require coenzymes; Oxidases and reductases, Monooxygenation and dioxygenation, Carboxylases and decarboxylases; Pyridoxyl dependent decarboxylases, Amino acid racemases, epimerases, aldose-ketose isomerases, cis-trans isomerases, Enzyme catalyzed elimination and addition reactions, Rearrangement reactions. Methods of improving enzyme stability.

Tools in recombinant DNA technology (10 L)

Nucleic acid structure and function, Chemical synthesis of DNA, Enzymes used in recombinant DNA technology and their applications, Vector systems, Cloning strategies, Analysis of cloned/recombinant DNA molecules.

Applications in recombinant DNA technology (07 L)

Application of recombinant DNA technology in medicine, forensic science and industry, Genetically modified organisms and foods.

Fermentation process (05 L)

Fermentation principle, Enzymatic reactions involved in fermentation, Microbial growth curve and kinetics, Basic functions of a fermenter, Application of fermentation in industries and carrying out fermentation in large scale processes, Purification and characterization of fermented products, Usage of fermentation end products.

Microbial products (09 L)

Commonly used microbes in industry, Metabolic pathways for the biosynthesis of microbial products (enzymes, antibiotics, organic acids, alkaloids etc), Over production of microbial products, Applications of microbes in industry, medicine, food, milk & milk products and beverages.