MOC 5002 : Separation and Characterization Techniques (2C)

MOC 5002 : Separation and Characterization Techniques (30L) - 2 credits

Separation methods (10 L)

Chromatogrpahic separation mechanisms, Chromatographic retention, Normal and reverse phase liquid chromatography: Capacity factor, selectivity, resolution, column efficiency, temperature programming and gradient elution, Qualitative and quantitative analytical methods, GC instrumentation and troubleshooting, HPLC instrumentation and troubleshooting, Chromatographic detectors.

Molecular spectroscopy – absorption (12 L)

Ultraviolet and visible spectroscopy, Infrared spectroscopy, Advanced ¹H and ¹³C NMR spectroscopy: Chemically and magnetically equivalent nuclei, long range coupling, first order versus complex spectra, introduction to 2D NMR (COSY, Hetcorr), uses of HMQC and HMBC,  Mass spectrometry: Fragmentation patterns of the common classes of compounds in electron ionization, different soft ionization methods (FAB, electrospray and MALDI), GC-MS, LC-MS and MS/MS techniques and their use in structure elucidation.

Molecular spectroscopy - emission (04 L)

Molecular fluorescence, phosphorescence and chemiluminescence, Theory of fluorescence and phosphorescence, Applications and photoluminescence methods: Steady state and time resolved methods, fluorescence quenching and enhancing reagents, fluorescence probes.

Atomic spectroscopy - absorption and emission (04 L)

Absorption of radiation by free atoms, Difficulties of the use of a continuous source of radiation, Hollow Cathode Lamp (HCL), Nebulizers. Flame and non-flame atomizers, Interferences in AAS and methods to eliminate them, Solvent effects, Matrix effect. Atomic emission spectroscopy, Precautions in using flame photometry.